Rapidly growing cultures of 3T3-L1 preadipocytes produce large quantities of
prostaglandins when they are either stimulated with the calcium ionophore A23187 or
incubated with arachidonic acid. The main prostaglandin produced under all conditions was
prostaglandin E 2. Prostaglandin production in response to ionophore stimulation or
incubation with arachidonic acid decreased markedly, however, as the cultures approached
confluence, were maintained in the confluent state, or were stimulated to differentiate …