A study of human T-cell lines generated from multiple sclerosis patients and controls by stimulation with peptides of myelin basic protein
MP Pender, PA Csurhes, RA Houghten… - Journal of …, 1996 - Elsevier
We generated T-cell lines from the peripheral blood of controls and of patients with multiple
sclerosis (MS) by stimulation with overlapping synthetic peptides representing the entire
sequences of all four isoforms of human myelin basic protein (MBP). The T-cell lines reacted
to a wide range of epitopes in the major isoforms of MBP and to epitopes that were present
only in the minor isoforms. Many MS patients and controls had T-cells responding to one or
more cryptic MBP epitopes, as indicated by the generation of a peptide-specific T-cell line (s) …
sclerosis (MS) by stimulation with overlapping synthetic peptides representing the entire
sequences of all four isoforms of human myelin basic protein (MBP). The T-cell lines reacted
to a wide range of epitopes in the major isoforms of MBP and to epitopes that were present
only in the minor isoforms. Many MS patients and controls had T-cells responding to one or
more cryptic MBP epitopes, as indicated by the generation of a peptide-specific T-cell line (s) …