High resolution preparative column chromatographic system for gangliosides using DEAE-Sephadex and a new porus silica, Iatrobeads
M Takashi, A Susumu, M Yoshitaka - … et Biophysica Acta (BBA)-Lipids and …, 1976 - Elsevier
A new high-resolution preparative column chromatographic system was developed for
efficient and rapid isolation of ganglioside molecular species. The system involved a
combination of ion-exchange and adsorption chromatographies using DEAE-Sephadex A-
25 and the newly developed, totally porous silica spheres, Iatrobeads. Using this system the
brain gangliosides, GM 1, GD 1a, GD 1b and GT 1 were obtained in high purity and in
milligram amounts, in a relatively short time, by simple procedures. The presence of a …
efficient and rapid isolation of ganglioside molecular species. The system involved a
combination of ion-exchange and adsorption chromatographies using DEAE-Sephadex A-
25 and the newly developed, totally porous silica spheres, Iatrobeads. Using this system the
brain gangliosides, GM 1, GD 1a, GD 1b and GT 1 were obtained in high purity and in
milligram amounts, in a relatively short time, by simple procedures. The presence of a …