[HTML][HTML] Diffusion-limited reaction of free nitric oxide with erythrocytes
X Liu, MJS Miller, MS Joshi… - Journal of biological …, 1998 - ASBMB
Concentration changes of nitric oxide (NO) were monitored using an NO-sensitive electrode
in phosphate-buffered saline (PBS) with either free oxyhemoglobin (oxyHb) or red blood
cells (RBCs). In aerated PBS, the half-life of 0.9 μm NO is greater than 4 min. NO is
undetectable (< 50 nm) when added to a solution of oxyHb because the reaction of NO with
oxyHb is rapid. The disappearance rate of NO in PBS containing RBCs is rapid, compared
with PBS, but it is much slower (by a factor of approximately 650) than with an equivalent …
in phosphate-buffered saline (PBS) with either free oxyhemoglobin (oxyHb) or red blood
cells (RBCs). In aerated PBS, the half-life of 0.9 μm NO is greater than 4 min. NO is
undetectable (< 50 nm) when added to a solution of oxyHb because the reaction of NO with
oxyHb is rapid. The disappearance rate of NO in PBS containing RBCs is rapid, compared
with PBS, but it is much slower (by a factor of approximately 650) than with an equivalent …