Oncogenic mutants of RON and MET receptor tyrosine kinases cause activation of the β-catenin pathway

A Danilkovitch-Miagkova, A Miagkov… - … and cellular biology, 2001 - Taylor & Francis
A Danilkovitch-Miagkova, A Miagkov, A Skeel, N Nakaigawa, B Zbar, EJ Leonard
Molecular and cellular biology, 2001Taylor & Francis
β-Catenin is an oncogenic protein involved in regulation of cell-cell adhesion and gene
expression. Accumulation of cellular β-catenin occurs in many types of human cancers. Four
mechanisms are known to cause increases in β-catenin: mutations of β-catenin,
adenomatous polyposis coli, or axin genes and activation of Wnt signaling. We report a new
cause of β-catenin accumulation involving oncogenic mutants of RON and MET receptor
tyrosine kinases (RTKs). Cells transfected with oncogenic RON or MET were characterized …
β-Catenin is an oncogenic protein involved in regulation of cell-cell adhesion and gene expression. Accumulation of cellular β-catenin occurs in many types of human cancers. Four mechanisms are known to cause increases in β-catenin: mutations of β-catenin, adenomatous polyposis coli, or axin genes and activation of Wnt signaling. We report a new cause of β-catenin accumulation involving oncogenic mutants of RON and MET receptor tyrosine kinases (RTKs). Cells transfected with oncogenic RON or MET were characterized by β-catenin tyrosine phosphorylation and accumulation; constitutive activation of a Tcf transcriptional factor; and increased levels of β-catenin/Tcf target oncogene proteins c-mycand cyclin D1. Interference with the β-catenin pathway reduced the transforming potential of mutated RON and MET. Activation of β-catenin by oncogenic RON and MET constitutes a new pathway, which might lead to cell transformation by these and other mutant growth factor RTKs.
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