[CITATION][C] [22] Isolation and assay of Pseudomonas aeruginosa alginate

TB May, AM Chakrabarty - Methods in enzymology, 1994 - Elsevier
Pseudomonas aeruginosa that are isolated from sputum of patients having cystic fibrosis
often have a mucoid appearance owing to the synthesis and secretion of an
exopolysaccharide. Linker and Jones 1, 2 first reported that this polysaccharide was similar
to alginate, a linear copolymer composed of fl-l, 4-1inked D-mannuronic acid and its C-5
epimer L-guluronic acid (Fig. 1). Alginate is a commercially important polymer obtained from
marine algae) Alginate has also been identified as a component of the slime layer of the …