[HTML][HTML] Biochemical, Structural, and Transglutaminase Substrate Properties Of Human Loricrin, the Major Epidermal Cornified Cell Envelope Protein (∗)
Loricrin is the major protein of the cornified cell envelope of terminally differentiated
epidermal keratinocytes which functions as a physical barrier. In order to understand its
properties and role in cornified cell envelope, we have expressed human loricrin from a full-
length cDNA clone in bacteria and purified it to homogeneity. We have also isolated loricrin
from newborn mouse epidermis. By circular dichroism and fluorescence spectroscopy, the in
vivo mouse and bacterially expressed human loricrins possess no α or β structure but have …
epidermal keratinocytes which functions as a physical barrier. In order to understand its
properties and role in cornified cell envelope, we have expressed human loricrin from a full-
length cDNA clone in bacteria and purified it to homogeneity. We have also isolated loricrin
from newborn mouse epidermis. By circular dichroism and fluorescence spectroscopy, the in
vivo mouse and bacterially expressed human loricrins possess no α or β structure but have …