[HTML][HTML] Characterization and quantitation of apolipoprotein B-100 by capillary electrophoresis
ID Cruzado, SL Cockrill, CJ McNeal… - Journal of lipid …, 1998 - ASBMB
The interaction of low density lipoproteins (LDL) with different surfactants was studied by
capillary electrophoresis (CE) and sucrose density gradient ultracentrifugation as part of
developing a method for quantitation of apoB-100 in serum. A mixture of surfactants
consisting of 70% sodium dodecyl sulfate (SDS), 25% sodium myristyl sulfate, and 5%
sodium cetyl sulfate was found to delipidate LDL particles more effectively than pure SDS or
sodium decyl sulfate. The delipidation products of LDL [apolipoprotein B-100 (apoB-100) …
capillary electrophoresis (CE) and sucrose density gradient ultracentrifugation as part of
developing a method for quantitation of apoB-100 in serum. A mixture of surfactants
consisting of 70% sodium dodecyl sulfate (SDS), 25% sodium myristyl sulfate, and 5%
sodium cetyl sulfate was found to delipidate LDL particles more effectively than pure SDS or
sodium decyl sulfate. The delipidation products of LDL [apolipoprotein B-100 (apoB-100) …