GM-CSF and oncogene mRNA stabilities are independently regulated in trans in a mouse monocytic tumor
GD Schuler, MD Cole - Cell, 1988 - cell.com
GD Schuler, MD Cole
Cell, 1988•cell.comRegulation of mRNA turnover has emerged as an important control point in lymphokine and
oncogene expression. We have studied a monocytic tumor in which activation of GM-CSF
expression results from the constitutive stabilization of the normally short-lived GMCSF
mRNA. Linkage of the germ-line 3'untranslated region of the GM-CSF gene to a neo reporter
gene demonstrated that mRNA stabilization is mediated by a tumor-specific frans-acting
factor (s), rather than by an alteration of the GM-CSF gene itself. Significantly, similar fusions …
oncogene expression. We have studied a monocytic tumor in which activation of GM-CSF
expression results from the constitutive stabilization of the normally short-lived GMCSF
mRNA. Linkage of the germ-line 3'untranslated region of the GM-CSF gene to a neo reporter
gene demonstrated that mRNA stabilization is mediated by a tumor-specific frans-acting
factor (s), rather than by an alteration of the GM-CSF gene itself. Significantly, similar fusions …
Summary
Regulation of mRNA turnover has emerged as an important control point in lymphokine and oncogene expression. We have studied a monocytic tumor in which activation of GM-CSF expression results from the constitutive stabilization of the normally short-lived GMCSF mRNA. Linkage of the germ-line 3’untranslated region of the GM-CSF gene to a neo reporter gene demonstrated that mRNA stabilization is mediated by a tumor-specific frans-acting factor (s), rather than by an alteration of the GM-CSF gene itself. Significantly, similar fusions of the c-myc and c-fos 3’untranslated regions to neo yielded mRNAs that turned over rapidly in all cells, including the tumor cells. These results demonstrate that AU-rich mRNA turnover signals are recognized differentially in trans within the same cell.
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