[HTML][HTML] Activation of Yeast Protein Kinase C by Rho1 GTPase (∗)
Y Kamada, H Qadota, CP Python, Y Anraku… - Journal of Biological …, 1996 - ASBMB
We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in
budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of
mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is
suppressed by overexpression of Pkc1. rho1 mutants are also impaired in pathway
activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with
Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP …
budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of
mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is
suppressed by overexpression of Pkc1. rho1 mutants are also impaired in pathway
activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with
Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP …