[HTML][HTML] Engineering the human thyrotropin receptor ectodomain from a non-secreted form to a secreted, highly immunoreactive glycoprotein that neutralizes …
GD Chazenbalk, JC Jaume, SM McLachlan… - Journal of Biological …, 1997 - ASBMB
Previous attempts to generate autoantibody-reactive, secreted thyrotropin receptor (TSHR)
ectodomain in mammalian cells have failed because of retention within the cell of material
with immature carbohydrate. We have overcome this difficulty by performing progressive
carboxyl-terminal truncations of the human TSHR ectodomain (418 amino acid residues
including signal peptide). Three ectodomain variants (TSHR-261, TSHR-289, and TSHR-
309) were truncated at residues 261, 289, and 309, respectively. Unlike the full ectodomain …
ectodomain in mammalian cells have failed because of retention within the cell of material
with immature carbohydrate. We have overcome this difficulty by performing progressive
carboxyl-terminal truncations of the human TSHR ectodomain (418 amino acid residues
including signal peptide). Three ectodomain variants (TSHR-261, TSHR-289, and TSHR-
309) were truncated at residues 261, 289, and 309, respectively. Unlike the full ectodomain …