Analysis of hyaluronan using biotinylated hyaluronan-binding proteins
CB Underhill, L Zhang - Developmental Biology Protocols, 2000 - Springer
CB Underhill, L Zhang
Developmental Biology Protocols, 2000•SpringerIn this chapter, we describe the preparation and use of b-PG, a biotinylated complex that
specifically binds hyaluronan (1, 2). The b-PG is derived from cartilage and consists of a
trypsin fragment of the proteoglycan core protein and one of the link proteins. Because of its
ability to bind to hyaluronan with high affinity and specificity, the b-PG agent has proved to
be useful in the histochemical localization of hyaluronan and its quantitative analysis by an
enzyme linked assay (1, 2).
specifically binds hyaluronan (1, 2). The b-PG is derived from cartilage and consists of a
trypsin fragment of the proteoglycan core protein and one of the link proteins. Because of its
ability to bind to hyaluronan with high affinity and specificity, the b-PG agent has proved to
be useful in the histochemical localization of hyaluronan and its quantitative analysis by an
enzyme linked assay (1, 2).
Abstract
In this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan (1,2). The b-PG is derived from cartilage and consists of a trypsin fragment of the proteoglycan core protein and one of the link proteins. Because of its ability to bind to hyaluronan with high affinity and specificity, the b-PG agent has proved to be useful in the histochemical localization of hyaluronan and its quantitative analysis by an enzyme linked assay (1,2).
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