Cell preparation for the identification of leukocytes

CC Stewart, SJ Stewart - Methods in Cell Biology, 1994 - Elsevier
Publisher Summary There are many problems and pitfalls that can be encountered when
labeling cells with antibodies. These problems need to be recognized and addressed when
they occur and solutions devised, if the data obtained are to be correctly interpreted. This
chapter focuses on these problems, their probable solutions, and several strategies for
staining cells with antibodies labeled with different colored fluorochromes. Advances in flow
cytometry instrumentation design and the availability of new fluorochromes and staining …