[HTML][HTML] Inactivating mutation of the mouse tissue inhibitor of metalloproteinases-2 (Timp-2) gene alters proMMP-2 activation
JJ Caterina, S Yamada, NCM Caterina… - Journal of Biological …, 2000 - ASBMB
To understand the biologic function of TIMP-2, a member of the tissue inhibitors of
metalloproteinases family, an inactivating mutation was introduced in the mouse Timp-
2gene by homologous recombination. Outbred homozygous mutants developed and
procreated indistinguishably from wild type littermates, suggesting that fertility, development,
and growth are not critically dependent on TIMP-2. Lack of functional TIMP-2, however,
dramatically altered the activation of proMMP-2 both in vivo andin vitro. Fully functional TIMP …
metalloproteinases family, an inactivating mutation was introduced in the mouse Timp-
2gene by homologous recombination. Outbred homozygous mutants developed and
procreated indistinguishably from wild type littermates, suggesting that fertility, development,
and growth are not critically dependent on TIMP-2. Lack of functional TIMP-2, however,
dramatically altered the activation of proMMP-2 both in vivo andin vitro. Fully functional TIMP …