[HTML][HTML] Antagonists of the receptor-G protein interface block Gi-coupled signal transduction
A Gilchrist, MR Mazzoni, B Dineen, A Dice… - Journal of Biological …, 1998 - ASBMB
The carboxyl terminus of heterotrimeric G protein α subunits plays an important role in
receptor interaction. We demonstrate that peptides corresponding to the last 11 residues of
Gα i1/2 or Gα o1 impair agonist binding to A 1 adenosine receptors, whereas Gα s or Gα t
peptides have no effect. Previously, by using a combinatorial library we identified a series of
Gα t peptide analogs that bind rhodopsin with high affinity (Martin, EL, Rens-Domiano, S.,
Schatz, PJ, and Hamm, HE (1996) J. Biol. Chem. 271, 361–366). Native Gα i1/2 peptide as …
receptor interaction. We demonstrate that peptides corresponding to the last 11 residues of
Gα i1/2 or Gα o1 impair agonist binding to A 1 adenosine receptors, whereas Gα s or Gα t
peptides have no effect. Previously, by using a combinatorial library we identified a series of
Gα t peptide analogs that bind rhodopsin with high affinity (Martin, EL, Rens-Domiano, S.,
Schatz, PJ, and Hamm, HE (1996) J. Biol. Chem. 271, 361–366). Native Gα i1/2 peptide as …