[HTML][HTML] Highly sensitive sequencing of the sulfated domains of heparan sulfate

CLR Merry, M Lyon, JA Deakin, JJ Hopwood… - Journal of Biological …, 1999 - ASBMB
The heparan sulfates (HS) are hypervariable linear polysaccharides that act as membrane
co-receptors for growth factors, chemokines, and extracellular matrix proteins. In most
instances, the molecular basis of protein recognition by HS is poorly understood. We have
sequenced 75% of the sulfated domains (S-domains) of fibroblast HS, including all of the
major ones. This analysis revealed tight coupling of N-and 2-O-sulfation and a low
frequency but precise positioning of 6-O-sulfates, which are required functional groups for …