Glutamate-induced cytotoxicity in PC12 pheochromocytoma cells: role of oxidation of phospholipids, glutathione and protein sulfhydryls revealed by bcl-2 transfection
Incubation of mock-transfected PC12 rat pheochromocytoma cells (PC12) for 2 h with
increasing concentrations of glutamate caused progressive loss of viability (eg, 67% with 15
mM glutamate). In contrast, the viability of bcl-2-transfected cells (PC12/bcl-2) was
unaffected by glutamate. Neither PC12 nor PC12/bcl-2 cells showed a significant incidence
of apoptosis in response to glutamate. Conventional phospholipid analysis by high-
performance TLC and phosphorous determination showed no significant changes in the …
increasing concentrations of glutamate caused progressive loss of viability (eg, 67% with 15
mM glutamate). In contrast, the viability of bcl-2-transfected cells (PC12/bcl-2) was
unaffected by glutamate. Neither PC12 nor PC12/bcl-2 cells showed a significant incidence
of apoptosis in response to glutamate. Conventional phospholipid analysis by high-
performance TLC and phosphorous determination showed no significant changes in the …