Platelet aggregation monitored in a 96 well microplate reader is useful for evaluation of platelet agonists and antagonists
B Bednar, C Condra, RJ Gould, TM Connolly - Thrombosis research, 1995 - Elsevier
Optimal conditions for a method to simultaneously measure aggregation in 96 samples
using a microplate reader were developed. The temperature of the assay was set at 25° C,
the optimal platelet concentration range was determined to be from 1–3× 108 per mL, the
assay volume was determined to be best at 100 μL and an agitation rate of setting# 5 on the
vortex was found to yield the most reliable aggregation response. After these initial assay
parameters were established, EC50 values for standard platelet agonists including ADP …
using a microplate reader were developed. The temperature of the assay was set at 25° C,
the optimal platelet concentration range was determined to be from 1–3× 108 per mL, the
assay volume was determined to be best at 100 μL and an agitation rate of setting# 5 on the
vortex was found to yield the most reliable aggregation response. After these initial assay
parameters were established, EC50 values for standard platelet agonists including ADP …