Mechanism-based inactivation of leukotriene A4 hydrolase during leukotriene B4 formation by human erythrocytes.
L Orning, DA Jones, FA Fitzpatrick - Journal of Biological Chemistry, 1990 - ASBMB
Evidence is presented in support of a mechanism-based (suicide) inactivation of leukotriene
A4 hydrolyase in intact human erythrocytes by leukotriene A4 and leukotriene A4 methyl
ester. Loss of enzymatic activity, accompanying leukotriene B4 formation, was proportional
to the substrate concentration. Inactivation was directly related to the amount of leukotriene
B4 formation: for several, different experimental protocols 50% loss of hydrolase activity
corresponded with formation of 10.3+/-2.1 microM leukotriene B4. The time course of …
A4 hydrolyase in intact human erythrocytes by leukotriene A4 and leukotriene A4 methyl
ester. Loss of enzymatic activity, accompanying leukotriene B4 formation, was proportional
to the substrate concentration. Inactivation was directly related to the amount of leukotriene
B4 formation: for several, different experimental protocols 50% loss of hydrolase activity
corresponded with formation of 10.3+/-2.1 microM leukotriene B4. The time course of …