Isolation and properties of intermediates in prostaglandin biosynthesis
DH Nugteren, E Hazelhof - Biochimica et Biophysica Acta (BBA)-Lipids and …, 1973 - Elsevier
Two intermediates could be isolated during the enzymic conversion of polyunsaturated fatty
acids into prostaglandin E using sheep vesicular glands. This indicates that this biosynthesis
proceeds in at least 3 consecutive enzymic conversions which now can be studied
separately: 2-fold oxygenation occurs, followed by reduction of the 15-hydroperoxy group
and enzymic isomerisation of the 9, 11-cyclic endoperoxide. It was found that the latter
isomerisation, which gives prostaglandin E, does not occur in many tissues and that the …
acids into prostaglandin E using sheep vesicular glands. This indicates that this biosynthesis
proceeds in at least 3 consecutive enzymic conversions which now can be studied
separately: 2-fold oxygenation occurs, followed by reduction of the 15-hydroperoxy group
and enzymic isomerisation of the 9, 11-cyclic endoperoxide. It was found that the latter
isomerisation, which gives prostaglandin E, does not occur in many tissues and that the …