[HTML][HTML] Endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression
M Michalak, K Burns, C Andrin, N Mesaeli… - Journal of Biological …, 1996 - ASBMB
Calreticulin is a ubiquitously expressed Ca 2+-binding protein of the endoplasmic reticulum
(ER), which inhibits DNA binding in vitro and transcriptional activation in vivo by steroid
hormone receptors. Transient transfection assays were carried out to investigate the effects
of different intracellular targeting of calreticulin on transactivation mediated by glucocorticoid
receptor. BSC40 cells were transfected with either calreticulin expression vector (ER form of
calreticulin) or calreticulin expression vector encoding calreticulin minus leader peptide …
(ER), which inhibits DNA binding in vitro and transcriptional activation in vivo by steroid
hormone receptors. Transient transfection assays were carried out to investigate the effects
of different intracellular targeting of calreticulin on transactivation mediated by glucocorticoid
receptor. BSC40 cells were transfected with either calreticulin expression vector (ER form of
calreticulin) or calreticulin expression vector encoding calreticulin minus leader peptide …