[HTML][HTML] Regulation of Integrin Affinity States through an NP XY Motif in the β Subunit Cytoplasmic Domain
TE O'Toole, J Ylanne, BM Culley - Journal of Biological Chemistry, 1995 - ASBMB
The ligand binding affinities of the integrins are regulated through their cytoplasmic
domains. To identify specific residues that are involved in this process, we have generated
mutants in the β 1 and β 3 tails and coexpressed them in Chinese hamster ovary cells with
constitutively active α subunits. These α subunits are chimera of extracellular and
transmembrane α IIb joined to the cytoplasmic domains of α 5, α 6A, or α 6B and confer an
energy-dependent high affinity state when expressed in Chinese hamster ovary cells. The …
domains. To identify specific residues that are involved in this process, we have generated
mutants in the β 1 and β 3 tails and coexpressed them in Chinese hamster ovary cells with
constitutively active α subunits. These α subunits are chimera of extracellular and
transmembrane α IIb joined to the cytoplasmic domains of α 5, α 6A, or α 6B and confer an
energy-dependent high affinity state when expressed in Chinese hamster ovary cells. The …