Intrahepatic accumulation of nitrotyrosine in chronic viral hepatitis is associated with histological severity of liver disease
C García-Monzón, PL Majano, I Zubia, P Sanz… - Journal of …, 2000 - Elsevier
Background/Aims: The toxicity of nitric oxide is thought to be engendered, at least in part, by
its reaction with superoxide yielding peroxynitrite, a potent oxidant that promotes the
formation of nitrotyrosine within cells and tissue lesions. In this study we assessed the
intrahepatic localization and distribution of the inducible nitric oxide synthase (iNOS) and
nitrotyrosine (NTY) in patients with viral and non-viral liver disease. Methods: We carried out
single and double immunostaining experiments on cryostat liver biopsy sections using …
its reaction with superoxide yielding peroxynitrite, a potent oxidant that promotes the
formation of nitrotyrosine within cells and tissue lesions. In this study we assessed the
intrahepatic localization and distribution of the inducible nitric oxide synthase (iNOS) and
nitrotyrosine (NTY) in patients with viral and non-viral liver disease. Methods: We carried out
single and double immunostaining experiments on cryostat liver biopsy sections using …