[HTML][HTML] Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation
N Kinto, M Iwamoto, M Enomoto-Iwamoto, S Noji… - FEBS letters, 1997 - Elsevier
N Kinto, M Iwamoto, M Enomoto-Iwamoto, S Noji, H Ohuchi, H Yoshioka, H Kataoka, Y Wada…
FEBS letters, 1997•ElsevierWe investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone
formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2
and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken
fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N).
The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also
significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular …
formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2
and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken
fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N).
The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also
significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular …
We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation. © 1997 Federation of European Biochemical Societies.
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