Identification of intimal subendothelial cells from human aorta in primary culture
VR Babaev, AS Antonov, OS Zacharova, YA Romanov… - Atherosclerosis, 1988 - Elsevier
Subendothelial cells (SEC) were obtained from the inner intimal layer of adult human aorta
by collagenase treatment. SEC were identified in primary culture either as smooth muscle
cells by staining with FITC-labeled antisera against human smooth muscle myosin or as
macrophages, foam cells and contaminating endothelial cells by their uptake of
malondialdehyde treated low density lipoproteins labeled with fluorescent dye 3, 3′-
dioctadecylindocarbocyanine. Between 1 and 5 days in culture, along with smooth muscle …
by collagenase treatment. SEC were identified in primary culture either as smooth muscle
cells by staining with FITC-labeled antisera against human smooth muscle myosin or as
macrophages, foam cells and contaminating endothelial cells by their uptake of
malondialdehyde treated low density lipoproteins labeled with fluorescent dye 3, 3′-
dioctadecylindocarbocyanine. Between 1 and 5 days in culture, along with smooth muscle …