Inactivation of the flagellin gene of Salmonella enterica serotype Enteritidis strongly reduces into differentiated Caco-2 cells
FJAM Van Asten, HG Hendriks… - FEMS Microbiology …, 2000 - academic.oup.com
FJAM Van Asten, HG Hendriks, JFJG Koninkx, BAM Van der Zeijst, W Gaastra
FEMS Microbiology Letters, 2000•academic.oup.comA nonflagellated mutant of Salmonella enterica serotype Enteritidis was constructed by
disrupting the flagellin gene (fliC). Northern blot analysis indicated that the mutation did not
affect expression of the downstream fliU gene. Infection experiments with differentiated Caco-
2 cells revealed that the mutant was about 50-fold less invasive than the wild-type strain,
while bacterial adherence was unaffected. Complementation of the mutant with an intact fliC
copy restored flagella formation and efficient bacterial. Our data demonstrate that the fliC …
disrupting the flagellin gene (fliC). Northern blot analysis indicated that the mutation did not
affect expression of the downstream fliU gene. Infection experiments with differentiated Caco-
2 cells revealed that the mutant was about 50-fold less invasive than the wild-type strain,
while bacterial adherence was unaffected. Complementation of the mutant with an intact fliC
copy restored flagella formation and efficient bacterial. Our data demonstrate that the fliC …
Abstract
A nonflagellated mutant of Salmonella enterica serotype Enteritidis was constructed by disrupting the flagellin gene (fliC). Northern blot analysis indicated that the mutation did not affect expression of the downstream fliU gene. Infection experiments with differentiated Caco-2 cells revealed that the mutant was about 50-fold less invasive than the wild-type strain, while bacterial adherence was unaffected. Complementation of the mutant with an intact fliC copy restored flagella formation and efficient bacterial. Our data demonstrate that the fliC gene of S. enterica serotype Enteritidis is essential for the of Caco-2 cells.
Oxford University Press