Type X collagen synthesis in human osteoarthritic cartilage. Indication of chondrocyte hypertrophy
K Von der Mark, T Kirsch, A Nerlich… - … : Official Journal of …, 1992 - Wiley Online Library
K Von der Mark, T Kirsch, A Nerlich, A Kuss, G Weseloh, K Glückert, H Stöss
Arthritis & Rheumatism: Official Journal of the American College …, 1992•Wiley Online LibraryObjective. To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA)
cartilage, using type X collagen as a specific marker. Methods. The biosynthesis of type X
collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with
3H‐proline, immunoprecipitation, and sodium dodecyl sulfate–polyacrylamide gel
electrophoresis of the synthesized collagens. Extracellular deposition of types X and II
collagen was analyzed immunohistochemically. Results. Immunostaining revealed an …
cartilage, using type X collagen as a specific marker. Methods. The biosynthesis of type X
collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with
3H‐proline, immunoprecipitation, and sodium dodecyl sulfate–polyacrylamide gel
electrophoresis of the synthesized collagens. Extracellular deposition of types X and II
collagen was analyzed immunohistochemically. Results. Immunostaining revealed an …
Abstract
Objective. To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA) cartilage, using type X collagen as a specific marker.
Methods. The biosynthesis of type X collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with 3H‐proline, immunoprecipitation, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis of the synthesized collagens. Extracellular deposition of types X and II collagen was analyzed immunohistochemically.
Results. Immunostaining revealed an irregular distribution of type X collagen, which was localized around chondrocyte clusters in fibrillated OA cartilage, but was absent from the noncalcified region of normal articular cartilage. Freshly isolated OA chondrocytes synthesized predominantly type X collagen, while control chondrocytes synthesized mostly type II collagen.
Conclusion. Our findings indicate focal premature chondrocyte differentiation to hypertrophic cells in OA cartilage.
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