Molecular cloning and sequence analysis of the mouse protein C inhibitor gene
M Zechmeister-Machhart, P Hufnagl, P Uhrin… - Gene, 1997 - Elsevier
M Zechmeister-Machhart, P Hufnagl, P Uhrin, I Korschineck, BR Binder, M Geiger
Gene, 1997•ElsevierThe gene encoding mouse protein C inhibitor (mPCI) was isolated and its nucleotide
sequence determined. Alignment of the genomic sequence with that of a cDNA obtained
from mouse testis revealed that the mPCI gene (like the human counterpart) is composed of
five exons and four introns with highly conserved exon/intron boundaries. It encodes a pre-
polypeptide of 405 amino acids, which shows 63% identity with human PCI (hPCI). The
putative reactive site is identical to that of hPCI from P5 to P3′, suggesting a similar …
sequence determined. Alignment of the genomic sequence with that of a cDNA obtained
from mouse testis revealed that the mPCI gene (like the human counterpart) is composed of
five exons and four introns with highly conserved exon/intron boundaries. It encodes a pre-
polypeptide of 405 amino acids, which shows 63% identity with human PCI (hPCI). The
putative reactive site is identical to that of hPCI from P5 to P3′, suggesting a similar …
The gene encoding mouse protein C inhibitor (mPCI) was isolated and its nucleotide sequence determined. Alignment of the genomic sequence with that of a cDNA obtained from mouse testis revealed that the mPCI gene (like the human counterpart) is composed of five exons and four introns with highly conserved exon/intron boundaries. It encodes a pre-polypeptide of 405 amino acids, which shows 63% identity with human PCI (hPCI). The putative reactive site is identical to that of hPCI from P5 to P3′, suggesting a similar protease specificity. Also the putative heparin binding sites and `hinge' regions are highly homologous in mouse and hPCI.
Elsevier