Cationic liposome-mediated RNA transfection.
RW Malone, PL Felgner… - Proceedings of the …, 1989 - National Acad Sciences
Proceedings of the National Academy of Sciences, 1989•National Acad Sciences
We have developed an efficient and reproducible method for RNA transfection, using a
synthetic cationic lipid, N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride
(DOTMA), incorporated into a liposome (lipofectin). Transfection of 10 ng to 5 micrograms of
Photinus pyralis luciferase mRNA synthesized in vitro into NIH 3T3 mouse cells yields a
linear response of luciferase activity. The procedure can be used to efficiently transfect RNA
into human, rat, mouse, Xenopus, and Drosophila cells. Using the RNA/lipofectin …
synthetic cationic lipid, N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride
(DOTMA), incorporated into a liposome (lipofectin). Transfection of 10 ng to 5 micrograms of
Photinus pyralis luciferase mRNA synthesized in vitro into NIH 3T3 mouse cells yields a
linear response of luciferase activity. The procedure can be used to efficiently transfect RNA
into human, rat, mouse, Xenopus, and Drosophila cells. Using the RNA/lipofectin …
We have developed an efficient and reproducible method for RNA transfection, using a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), incorporated into a liposome (lipofectin). Transfection of 10 ng to 5 micrograms of Photinus pyralis luciferase mRNA synthesized in vitro into NIH 3T3 mouse cells yields a linear response of luciferase activity. The procedure can be used to efficiently transfect RNA into human, rat, mouse, Xenopus, and Drosophila cells. Using the RNA/lipofectin transfection procedure, we have analyzed the role of capping and beta-globin 5' and 3' untranslated sequences on the translation efficiency of luciferase RNA synthesized in vitro. Following transfection of NIH 3T3 cells, capped mRNAs with beta-globin untranslated sequences produced at least 1000-fold more luciferase protein than mRNAs lacking these elements.
National Acad Sciences