[HTML][HTML] Differential pharmacological properties and signal transduction of the sphingosine 1-phosphate receptors EDG-1, EDG-3, and EDG-5
N Ancellin, T Hla - Journal of Biological Chemistry, 1999 - ASBMB
Sphingosine 1-phosphate (SPP) is a potent lipid mediator released upon cellular activation.
In this report, pharmacological properties of the three G-protein-coupled receptors (GPCRs)
for SPP, EDG-1,-3, and-5 are characterized using aXenopus oocyte expression system,
which lacks endogenous SPP receptors. Microinjection of the EDG-3 and EDG-5 but not
EDG-1 mRNA conferred SPP-responsive intracellular calcium transients; however, the EDG-
5 response was quantitatively much less. Co-expression of EDG-1 receptor with the chimeric …
In this report, pharmacological properties of the three G-protein-coupled receptors (GPCRs)
for SPP, EDG-1,-3, and-5 are characterized using aXenopus oocyte expression system,
which lacks endogenous SPP receptors. Microinjection of the EDG-3 and EDG-5 but not
EDG-1 mRNA conferred SPP-responsive intracellular calcium transients; however, the EDG-
5 response was quantitatively much less. Co-expression of EDG-1 receptor with the chimeric …