The vasoinhibitory activity of bovine chromogranin A fragment (vasostatin) and its independence of extracellular calcium in isolated segments of human blood vessels
S Aardal, KB Helle - Regulatory peptides, 1992 - Elsevier
Endothelium-independent vasoconstrictor responses in isolated segments of human internal
thoracic artery (ITA) and saphenous vein (SV) were used as a bioassay system for the
vasoinhibitory activity of bovine chromogranin A (CGA). Preincubation with vasostatin (0.8
μg/ml), containing the N-terminal domain of CGA,(CGA1–76, CGA1–113 and CGA1− 143ff),
inhibited the contractile responses evoked by 80 mM K+, 2.6 μM noradrenaline (NA), or 65
nM endothelin-1 (ET-1) in Ca2+-free solution in SV but not in ITA. The results demonstrate a …
thoracic artery (ITA) and saphenous vein (SV) were used as a bioassay system for the
vasoinhibitory activity of bovine chromogranin A (CGA). Preincubation with vasostatin (0.8
μg/ml), containing the N-terminal domain of CGA,(CGA1–76, CGA1–113 and CGA1− 143ff),
inhibited the contractile responses evoked by 80 mM K+, 2.6 μM noradrenaline (NA), or 65
nM endothelin-1 (ET-1) in Ca2+-free solution in SV but not in ITA. The results demonstrate a …