A critical role for the peroxisome proliferator-activated receptor α (PPARα) in the cellular fasting response: the PPARα-null mouse as a model of fatty acid oxidation …

TC Leone, CJ Weinheimer… - Proceedings of the …, 1999 - National Acad Sciences
TC Leone, CJ Weinheimer, DP Kelly
Proceedings of the National Academy of Sciences, 1999National Acad Sciences
We hypothesized that the lipid-activated transcription factor, the peroxisome proliferator-
activated receptor α (PPARα), plays a pivotal role in the cellular metabolic response to
fasting. Short-term starvation caused hepatic steatosis, myocardial lipid accumulation, and
hypoglycemia, with an inadequate ketogenic response in adult mice lacking PPARα
(PPARα−/−), a phenotype that bears remarkable similarity to that of humans with genetic
defects in mitochondrial fatty acid oxidation enzymes. In PPARα+/+ mice, fasting induced the …
We hypothesized that the lipid-activated transcription factor, the peroxisome proliferator-activated receptor α (PPARα), plays a pivotal role in the cellular metabolic response to fasting. Short-term starvation caused hepatic steatosis, myocardial lipid accumulation, and hypoglycemia, with an inadequate ketogenic response in adult mice lacking PPARα (PPARα−/−), a phenotype that bears remarkable similarity to that of humans with genetic defects in mitochondrial fatty acid oxidation enzymes. In PPARα+/+ mice, fasting induced the hepatic and cardiac expression of PPARα target genes encoding key mitochondrial (medium-chain acyl-CoA dehydrogenase, carnitine palmitoyltransferase I) and extramitochondrial (acyl-CoA oxidase, cytochrome P450 4A3) enzymes. In striking contrast, the hepatic and cardiac expression of most PPARα target genes was not induced by fasting in PPARα−/− mice. These results define a critical role for PPARα in a transcriptional regulatory response to fasting and identify the PPARα−/− mouse as a potentially useful murine model of inborn and acquired abnormalities of human fatty acid utilization.
National Acad Sciences