Imaging system for three-dimensional mapping of cerebrocortical capillary networks in vivo
AG Hudetz, AS Greene, G Fehér, DE Knuese… - Microvascular …, 1993 - Elsevier
A personal computer-based image analysis system was developed for the three-
dimensional mapping of cerebrocortical capillary networks from intravital video recordings.
Capillary circulation in the parietal cortex of anesthetized rats was visualized to 70 μm depth
using a closed cranial window preparation and epifluorescent, intensified video microscopy.
The circulation was video recorded during slow (0.1 μm/frame) scanning of the focal plane
(depth of field= 10 μm) through regions of interest. Capillary networks were traced from still …
dimensional mapping of cerebrocortical capillary networks from intravital video recordings.
Capillary circulation in the parietal cortex of anesthetized rats was visualized to 70 μm depth
using a closed cranial window preparation and epifluorescent, intensified video microscopy.
The circulation was video recorded during slow (0.1 μm/frame) scanning of the focal plane
(depth of field= 10 μm) through regions of interest. Capillary networks were traced from still …