Molecular cloning of a human protein that binds to the retinoblastoma protein and chromosomal mapping
M Saijo, Y Sakai, T Kishino, N Niikawa, Y Matsuura… - Genomics, 1995 - Elsevier
M Saijo, Y Sakai, T Kishino, N Niikawa, Y Matsuura, K Morino, K Tamai, Y Taya
Genomics, 1995•ElsevierWe have isolated distinct clones for cellular proteins that bind to the retinoblastoma protein
by direct screening of cDNA expression libraries using purified pRB as a probe. The total
nucleotide sequence of one of these clones, RBQ-3, was determined and found to encode a
protein of 66 kDa localized in the nucleus. The RBQ-3 preferentially binds to
underphosphorylated pRB. The region used for binding to this protein was mapped to the
E1A-binding pocket B of pRB, which has sequence similarity to the general transcription …
by direct screening of cDNA expression libraries using purified pRB as a probe. The total
nucleotide sequence of one of these clones, RBQ-3, was determined and found to encode a
protein of 66 kDa localized in the nucleus. The RBQ-3 preferentially binds to
underphosphorylated pRB. The region used for binding to this protein was mapped to the
E1A-binding pocket B of pRB, which has sequence similarity to the general transcription …
We have isolated distinct clones for cellular proteins that bind to the retinoblastoma protein by direct screening of cDNA expression libraries using purified pRB as a probe. The total nucleotide sequence of one of these clones, RBQ-3, was determined and found to encode a protein of 66 kDa localized in the nucleus. The RBQ-3 preferentially binds to underphosphorylated pRB. The region used for binding to this protein was mapped to the E1A-binding pocket B of pRB, which has sequence similarity to the general transcription factor TFIIB. We have mapped the gene to 1q32 using polymerase chain reaction analysis on a human-hamster hybrid cell panel and chromosomal fluorescence in situ hybridization.
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