Mediation of Epstein-Barr Virus EBNA2 Transactivation by Recombination Signal-Binding Protein Jκ
T Henkel, PD Ling, SD Hayward, MG Peterson - Science, 1994 - science.org
T Henkel, PD Ling, SD Hayward, MG Peterson
Science, 1994•science.orgThe Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear
antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the
immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not
bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-
promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact
with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene …
antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the
immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not
bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-
promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact
with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene …
The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene rearrangement, RBPJκ. Contrary to previous reports, CBF1-RBPJκ did not bind to the recombination signal sequences but instead bound to sites in the EBV C-promoter and in the CD23 promoter.
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