Tyrosine phosphorylation of an SH2‐containing protein tyrosine phosphatase is coupled to platelet thrombin receptor via a pertussis toxin‐sensitive heterotrimeric G …

RY Li, F Gaits, A Ragab, JM Ragab‐Thomas… - The EMBO …, 1995 - embopress.org
RY Li, F Gaits, A Ragab, JM Ragab‐Thomas, H Chap
The EMBO journal, 1995embopress.org
SH‐PTP1 is a protein tyrosine phosphatase (PTP) predominantly expressed in
haematopoietic cells and containing two src homology‐2 (SH2) domains. Here we report
that SH‐PTP1 is phosphorylated on both serine and tyrosine residues in response to
thrombin or phorbol myristate acetate (PMA), which increased by 60 and 40%, respectively,
SH‐PTP1 activity. Thrombin‐induced phosphorylation of SH‐PTP1 is an early signalling
event (maximal within 10 s) involving neither integrin signalling, nor calcium, nor release of …
SH‐PTP1 is a protein tyrosine phosphatase (PTP) predominantly expressed in haematopoietic cells and containing two src homology‐2 (SH2) domains. Here we report that SH‐PTP1 is phosphorylated on both serine and tyrosine residues in response to thrombin or phorbol myristate acetate (PMA), which increased by 60 and 40%, respectively, SH‐PTP1 activity. Thrombin‐induced phosphorylation of SH‐PTP1 is an early signalling event (maximal within 10 s) involving neither integrin signalling, nor calcium, nor release of ADP or thromboxane A2. Moreover, in contrast with PMA, the effect of thrombin on the tyrosine phosphorylation of SH‐PTP1 was hardly affected by GF109203X, a specific protein kinase C (PKC) inhibitor. Finally, phosphorylation of SH‐PTP1 could be provoked in permeabilized platelets by thrombin or GTP gamma S. This was abolished by pertussis toxin, the specificity of this effect being verified with the megakaryocytic cell line Dami cell. Our data thus identify SH‐PTP1 as an in vivo substrate of a putative protein tyrosine kinase linked to the thrombin receptor by a Gi protein. This might offer some clue to unravel the mechanism of thrombin not only in platelets but also in nucleated cells, where its mitogenic effect is known to involve pertussis toxin‐sensitive G‐proteins, tyrosine phosphorylation and the ras pathway.
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