[HTML][HTML] Expression Cloning and Molecular Characterization of HAS Protein, a Eukaryotic Hyaluronan Synthase (∗)
N Itano, K Kimata - Journal of Biological Chemistry, 1996 - ASBMB
We developed a mammalian transient expression system to isolate cDNA clones that
determine hyaluronan expression. HAS-, a mouse mammary carcinoma mutant cell line,
which is defective in hyaluronan synthase activity, was first established and used as a
recipient for the expression cloning. One cloned cDNA that overcame the deficiency was
isolated. The cDNA termed HAS contains an open reading frame of 1749 base pairs
encoding a new protein of 583 amino acids. Homology analysis of the amino acid sequence …
determine hyaluronan expression. HAS-, a mouse mammary carcinoma mutant cell line,
which is defective in hyaluronan synthase activity, was first established and used as a
recipient for the expression cloning. One cloned cDNA that overcame the deficiency was
isolated. The cDNA termed HAS contains an open reading frame of 1749 base pairs
encoding a new protein of 583 amino acids. Homology analysis of the amino acid sequence …