An in vitro model for the study of acute release of von Willebrand factor from human endothelial cells
F Booth, MJ Allington… - British journal of …, 1987 - Wiley Online Library
F Booth, MJ Allington, SA Cederholm‐Williams
British journal of haematology, 1987•Wiley Online LibraryAn in vitro model is described which utilizes human umbilical vein endothelial cells cultured
on plastic microcarrier spheres and perfused with serum‐free medium. This model was used
to study the acute release of von Willebrand factor following stimulation of the cells with
putative agonists. Thrombin, plasmin and interleukin‐1 were found to release von
Willebrand factor. Adrenaline and bradykinin also stimulated release but only at high
dosage. 1‐desamino‐8‐d‐arginine vasopressin (DDAVP) was inactive.
on plastic microcarrier spheres and perfused with serum‐free medium. This model was used
to study the acute release of von Willebrand factor following stimulation of the cells with
putative agonists. Thrombin, plasmin and interleukin‐1 were found to release von
Willebrand factor. Adrenaline and bradykinin also stimulated release but only at high
dosage. 1‐desamino‐8‐d‐arginine vasopressin (DDAVP) was inactive.
Summary. An in vitro model is described which utilizes human umbilical vein endothelial cells cultured on plastic microcarrier spheres and perfused with serum‐free medium. This model was used to study the acute release of von Willebrand factor following stimulation of the cells with putative agonists. Thrombin, plasmin and interleukin‐1 were found to release von Willebrand factor. Adrenaline and bradykinin also stimulated release but only at high dosage. 1‐desamino‐8‐d‐arginine vasopressin (DDAVP) was inactive.
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