[HTML][HTML] The Two Nucleotide-binding Domains of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Have Distinct Functions in Controlling Channel …
MR Carson, SM Travis, MJ Welsh - Journal of Biological Chemistry, 1995 - ASBMB
The cystic fibrosis transmembrane conductance regulator (CFTR) Cl-channel contains two
cytoplasmic nucleotide-binding domains (NBDs). After phosphorylation of the R domain,
ATP interacts with the NBDs to regulate channel activity. To learn how the NBDs regulate
channel function, we used the patch-clamp technique to study CFTR and variants which
contained site-directed mutations in the conserved Walker A motif lysine residues in either
NBD1 (K464A), NBD2 (K1250A and K1250M), or both NBDs simultaneously …
cytoplasmic nucleotide-binding domains (NBDs). After phosphorylation of the R domain,
ATP interacts with the NBDs to regulate channel activity. To learn how the NBDs regulate
channel function, we used the patch-clamp technique to study CFTR and variants which
contained site-directed mutations in the conserved Walker A motif lysine residues in either
NBD1 (K464A), NBD2 (K1250A and K1250M), or both NBDs simultaneously …