Interleukin-4 inhibits bone resorption and acutely increases cytosolic Ca2+ in murine osteoclasts.
C Bizzarri, A Shioi, SL Teitelbaum, J Ohara… - Journal of Biological …, 1994 - ASBMB
Interleukin-4 (IL-4) is an immune cytokine recently shown to inhibit bone resorption. To
determine whether IL-4 directly acts on osteoclasts, we have analyzed its effect on cytosolic
calcium concentration [Ca2+] i and bone resorptive function of murine osteoclastic cells
generated from bone marrow/stromal cell co-cultures. IL-4 exposure induced an immediate
and sustained increase in [Ca2+] i that remained elevated for at least 10 min. This IL-4 effect
was dose-dependent, with the maximal effect (209+/-15% of baseline, n= 16) at 200 units/ml …
determine whether IL-4 directly acts on osteoclasts, we have analyzed its effect on cytosolic
calcium concentration [Ca2+] i and bone resorptive function of murine osteoclastic cells
generated from bone marrow/stromal cell co-cultures. IL-4 exposure induced an immediate
and sustained increase in [Ca2+] i that remained elevated for at least 10 min. This IL-4 effect
was dose-dependent, with the maximal effect (209+/-15% of baseline, n= 16) at 200 units/ml …