Immunological studies of human constitutive cyclooxygenase (COX-1) using enzyme immunometric assay
C Créminon, Y Frobert, A Habib, J Maclouf… - … et Biophysica Acta (BBA …, 1995 - Elsevier
Polyclonal antisera and six distinct monoclonal antibodies (mAbs) were raised against
constitutive cyclooxygenase (COX-1) purified from ram seminal vesicles. Immunoblotting
experiments revealed that the polyclonal antisera and 4 of the mAbs strongly recognized
human COX in platelet extracts. Different two-site immunometric assays of ram COX-1 were
established using different combinations of mAbs. The assays were performed in 96-well
microtiter plates coated with one mAb, with another mAb (covalently labeled with …
constitutive cyclooxygenase (COX-1) purified from ram seminal vesicles. Immunoblotting
experiments revealed that the polyclonal antisera and 4 of the mAbs strongly recognized
human COX in platelet extracts. Different two-site immunometric assays of ram COX-1 were
established using different combinations of mAbs. The assays were performed in 96-well
microtiter plates coated with one mAb, with another mAb (covalently labeled with …