T-lymphocyte interleukin 2-dependent tyrosine protein kinase signal transduction involves the activation of p56lck.

ID Horak, RE Gress, PJ Lucas… - Proceedings of the …, 1991 - National Acad Sciences
ID Horak, RE Gress, PJ Lucas, EM Horak, TA Waldmann, JB Bolen
Proceedings of the National Academy of Sciences, 1991National Acad Sciences
Addition of interleukin 2 (IL-2) to IL-2-dependent T cells results in tyrosine protein kinase
signal transduction events even though the IL-2 receptor alpha and beta chains lack intrinsic
enzymatic activity. Here we report that addition of IL-2 to IL-2-dependent human T cells
transiently stimulates the specific activity of p56lck, a member of the src family of nonreceptor
tyrosine protein kinases expressed at high levels in T lymphocytes. The ability of IL-2 to
induce p56lck activation was found to be independent of the capacity of p56lck to associate …
Addition of interleukin 2 (IL-2) to IL-2-dependent T cells results in tyrosine protein kinase signal transduction events even though the IL-2 receptor alpha and beta chains lack intrinsic enzymatic activity. Here we report that addition of IL-2 to IL-2-dependent human T cells transiently stimulates the specific activity of p56lck, a member of the src family of nonreceptor tyrosine protein kinases expressed at high levels in T lymphocytes. The ability of IL-2 to induce p56lck activation was found to be independent of the capacity of p56lck to associate with either CD4 or CD8. Following IL-2 treatment, p56lck was found to undergo serine/threonine phosphorylation modifications that resulted in altered mobility of the lck gene product on polyacrylamide gels. These observations raise the possibility that p56lck participates in IL-2-mediated signal transduction events in T cells.
National Acad Sciences