Two-dimensional analysis of interleukin 2-regulated tyrosine kinase activation mediated by the p70-75 β subunit of the interleukin 2 receptor
WL Farrar, DK Ferris - Journal of Biological Chemistry, 1989 - ASBMB
The proliferation of activated T lymphocytes is dependent on the interaction of the
polypeptide growth factor interleukin 2 (IL 2) with its heterodimeric receptor, which consists
of a p55 α subunit and a p70-75 β subunit. Previously, it was shown that IL 2 stimulates rapid
serine phosphorylation of several membrane and cytysolic proteins. Here, using anti-
phosphotyrosine antibodies to purify phosphotyrosyl proteins and two-dimensional gel
analysis, we show that IL 2 stimulates rapid tyrosine phosphorylation of a variety of cellular …
polypeptide growth factor interleukin 2 (IL 2) with its heterodimeric receptor, which consists
of a p55 α subunit and a p70-75 β subunit. Previously, it was shown that IL 2 stimulates rapid
serine phosphorylation of several membrane and cytysolic proteins. Here, using anti-
phosphotyrosine antibodies to purify phosphotyrosyl proteins and two-dimensional gel
analysis, we show that IL 2 stimulates rapid tyrosine phosphorylation of a variety of cellular …