A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration
W Falk, RH Goodwin Jr, EJ Leonard - Journal of immunological methods, 1980 - Elsevier
We designed a 48-well chemotaxis chamber to minimize manipulation time and amount of
material required by the larger blindwell or Boyden chemotaxis chamber. Cell and
chemoattractant dose-response curves showed that results were comparable to or better
than those obtained with blindwell chambers. The volume of chemoattractant per well is 25
μl; the number of cells can be as low as 10,000. The time needed for setting up this multiwell
unit and for staining the membrane filter sheet is negligible. Combined with the use of an …
material required by the larger blindwell or Boyden chemotaxis chamber. Cell and
chemoattractant dose-response curves showed that results were comparable to or better
than those obtained with blindwell chambers. The volume of chemoattractant per well is 25
μl; the number of cells can be as low as 10,000. The time needed for setting up this multiwell
unit and for staining the membrane filter sheet is negligible. Combined with the use of an …