Insulin and intracellular calcium responsiveness to glucagon-like peptide-1 and pituitary adenylate cyclase-activating peptide by dispersed adult porcine islet cells1
AM Davalli, F Bertuzzi, C Meoni, L Scaglia… - …, 1999 - journals.lww.com
AM Davalli, F Bertuzzi, C Meoni, L Scaglia, C Socci, G Pozza, AE Pontiroli
Transplantation, 1999•journals.lww.comBackground. There is a great need to learn more about porcine islet physiology because
porcine islets represent a promising source of xenogeneic tissue for β-cell replacement
therapy in humans. Methods. We evaluated the effects of two important physiological
regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate
cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++] i)
by adult porcine islet cells. Results. Exposure to GLP-1 and PACAP significantly potentiated …
porcine islets represent a promising source of xenogeneic tissue for β-cell replacement
therapy in humans. Methods. We evaluated the effects of two important physiological
regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate
cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++] i)
by adult porcine islet cells. Results. Exposure to GLP-1 and PACAP significantly potentiated …
Abstract
Background.
There is a great need to learn more about porcine islet physiology because porcine islets represent a promising source of xenogeneic tissue for β-cell replacement therapy in humans.
Methods.
We evaluated the effects of two important physiological regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++] i) by adult porcine islet cells.
Results.
Exposure to GLP-1 and PACAP significantly potentiated glucose-induced insulin release and improved the sensitivity to glucose as a secretagogue. About 70% of cells stimulated with 20 mmol/L glucose alone showed an increase in [Ca++] i, whereas the addition of GLP-1 and PACAP induced [Ca++] i increases in 86% and 93% of cells, respectively.
Conclusions.
The good insulin and [Ca++] i responsiveness of porcine islet cells to both GLP-1 and PACAP provides an additional proof of their suitability for transplantation.
Lippincott Williams & Wilkins