Construction of DNA sequences complementary to rat. alpha. 1 and. alpha. 2 collagen mRNA and their use in studying the regulation of type I collagen synthesis by 1 …

C Genovese, D Rowe, B Kream - Biochemistry, 1984 - ACS Publications
C Genovese, D Rowe, B Kream
Biochemistry, 1984ACS Publications
C. Genovese, D. Rowe,* andB. Kream abstract: Type I collagen mRNA from fetal rat calvaria
was used as a template for the synthesis of a cDNA that was subsequently inserted in the
Pstl site of the plasmic vector pBR322 and cloned. Three recombinant plasmids containing
type I collagen specific sequences were characterized: pc^ Rl is 1600 bp and spans
approximately 500 amino acid residues within the triple helicalregion of a,(I) and patR2 is
900 bp in size and covers the entire 3'noncoding and about half of the C-terminal propeptide …
C. Genovese, D. Rowe,* andB. Kream abstract: Type I collagen mRNA from fetal rat calvaria was used as a template for the synthesis of a cDNA that was subsequently inserted in the Pstl site of the plasmic vector pBR322 and cloned. Three recombinant plasmids containing type I collagen specific sequences were characterized: pc^ Rl is 1600 bp and spans approximately 500 amino acid residues within the triple helicalregion of a,(I) and patR2 is 900 bp in size and covers the entire 3'noncoding and about half of the C-terminal propeptide region of a^ I) collagen mRNA. The third recombinant pa2R2 is 1500bp and contains a2 (I) sequences specific for the entire 3'noncoding and C-terminal propeptide region. Partial nucleic acid sequence data revealed that thedecreasing order of amino acid and nucleotide homology to similar regions of the rat cDNA was mouse> human> chick. Northern hybridization of mRNA afterelectro-phoresis in 0.8% agarose revealed two distinctly different
ACS Publications