[HTML][HTML] Membrane targeting and determination of transmembrane topology of the human vasopressin V2 receptor
R Schülein, C Rutz, W Rosenthal - Journal of Biological Chemistry, 1996 - ASBMB
The human vasopressin V2 receptor belongs to the large family of G-protein-coupled
receptors, which possess seven transmembrane helices, an extracellular N terminus and an
intracellular C terminus. We have determined the sequence requirements of the V2 receptor
for membrane insertion and correct topology for the inner membrane of Escherichia coli with
the PhoA/LacZ gene fusion system. In addition, we have studied the signals for its
membrane insertion and correct topology for the membrane of the endoplasmic reticulum of …
receptors, which possess seven transmembrane helices, an extracellular N terminus and an
intracellular C terminus. We have determined the sequence requirements of the V2 receptor
for membrane insertion and correct topology for the inner membrane of Escherichia coli with
the PhoA/LacZ gene fusion system. In addition, we have studied the signals for its
membrane insertion and correct topology for the membrane of the endoplasmic reticulum of …