Activation of the alternative pathway of complement by microcrystalline cholesterol
P Hasselbacher, JL Hahn - Atherosclerosis, 1980 - Elsevier
Microcrystalline cholesterol in either the anhydrous or monohydrate form was a potent
activator of the alternative pathway of complement as measured by the electrophoretic
conversion (crossed immunoelectrophoresis) of C3 and properdin factor B. Chelation with
0.01 M ethylene-diaminetetraacetate (EDTA) completely eliminated conversion, but 0.01 M
ethyleneglycol tetra-acetate (EGTA) had little or no effect. The magnitude of activation by
cholesterol crystals was similar to that by zymosan, heat-aggregated IgG, or crystals of …
activator of the alternative pathway of complement as measured by the electrophoretic
conversion (crossed immunoelectrophoresis) of C3 and properdin factor B. Chelation with
0.01 M ethylene-diaminetetraacetate (EDTA) completely eliminated conversion, but 0.01 M
ethyleneglycol tetra-acetate (EGTA) had little or no effect. The magnitude of activation by
cholesterol crystals was similar to that by zymosan, heat-aggregated IgG, or crystals of …