[HTML][HTML] Cellular and molecular barriers to gene transfer by a cationic lipid
J Zabner, AJ Fasbender, T Moninger… - Journal of Biological …, 1995 - ASBMB
Cationic lipids are widely used for gene transfer in vitro and show promise as a vector for in
vivo gene therapy applications. However, there is limited understanding of the cellular and
molecular mechanisms involved. We investigated the individual steps in cationic lipid-
mediated gene transfer to cultured cell lines. We used DMRIE/DOPE (a 1: 1 mixture of N-[1-
(2, 3-dimyristyloxy) propyl]-N, N-dimethyl-N-(2-hydroxyethyl) ammonium bromide (DMRIE)
and dioleoyl phosphatidylethanolamine (DOPE)) as a model lipid because of its efficacy and …
vivo gene therapy applications. However, there is limited understanding of the cellular and
molecular mechanisms involved. We investigated the individual steps in cationic lipid-
mediated gene transfer to cultured cell lines. We used DMRIE/DOPE (a 1: 1 mixture of N-[1-
(2, 3-dimyristyloxy) propyl]-N, N-dimethyl-N-(2-hydroxyethyl) ammonium bromide (DMRIE)
and dioleoyl phosphatidylethanolamine (DOPE)) as a model lipid because of its efficacy and …