[HTML][HTML] Enzymatically Deacylated Lipopolysaccharide (LPS) Can Antagonize LPS at Multiple Sites in the LPS Recognition Pathway (∗)
RL Kitchens, RS Munford - Journal of Biological Chemistry, 1995 - ASBMB
Like other tetraacyl partial structures of lipopolysaccharide (LPS) and lipid A, LPS that has
been partially deacylated by acyloxyacyl hydrolase can inhibit LPS-induced responses in
human cells. To identify the site (s) of inhibition in the LPS recognition pathway, we analyzed
the apparent binding affinities and interactions of 3 H-labeled enzymatically deacylated LPS
(dLPS) and [3 H] LPS with CD14, the LPS receptor, on THP-1 cells. Using (i) incubation
conditions that prevented ligand internalization and (ii) defined concentrations of LPS …
been partially deacylated by acyloxyacyl hydrolase can inhibit LPS-induced responses in
human cells. To identify the site (s) of inhibition in the LPS recognition pathway, we analyzed
the apparent binding affinities and interactions of 3 H-labeled enzymatically deacylated LPS
(dLPS) and [3 H] LPS with CD14, the LPS receptor, on THP-1 cells. Using (i) incubation
conditions that prevented ligand internalization and (ii) defined concentrations of LPS …